New York - Apr 30th, 2018 - Creative Proteomics, as one of the most professional protein identification platform in the world, dedicating to providing multiple types of microarray analysis methods, including glycan-related microarray Assay, such as Lectin Microarray Assay, Glyco-gene Microarray Assay, Glycan Microarray Assay, Microbial Glycan Microarray Assay and Glycopeptide Microarray Assay.
Brief Introduction to Microarray Assay
Molecular biology research has been developed through the techniques developed for achieving them. It is impossible to study a large number of genes just with traditional methods. DNA microarrays are such a technology that enables researchers to investigate and solve problems that were used to be considered untraceable. Researchers can rapidly and efficiently analyze the expression of many genes in a single reaction.
A typical microarray experiment involves the hybridization of a mRNA molecule to its originating DNA template, and many DNA samples are used to build the array. The amount of mRNA bound to each site on the array indicates the level of expression of various genes. There may be thousands of data, collecting all of them and generate a profile for gene expression in the cells.
About Microarray technology
The array is an ordered arrangement of samples where the matching of known and unknown DNA samples is achieved based on the base pairing rules. Array experiments utilize commonly used assay systems, such as microplates or standard blot membranes. Sample sites are typically less than 200 microns in diameter and typically contain thousands of points.
Thousands of spot samples called probes (with known identities) are mounted on solid supports (microscope glass slides or silicon or nylon membranes). These spots can be DNA, cDNA or oligonucleotides, which are used to determine complementary binding of unknown sequences, allowing parallel analysis of gene expression and gene discovery. Experiments on a single DNA chip can provide information on thousands of genes at the same time.
Types of Microarray
Depending on the type of immobilized sample used and the information obtained, microarray experiments can be classified into three patterns:
1. Microarray expression analysis: In this experimental setup, cDNA from mRNA of a known gene was fixed. The sample has genes from normal and diseased tissues. If the gene is over-expressed in a disease state, spots with higher intensity of the diseased tissue gene are obtained. This expression pattern is then compared to the expression pattern of the gene responsible for the disease.
2. Microarrays for mutation analysis: For this analysis, the researchers used gDNA. These genes may differ by less than one nucleotide base.
A single base difference between two sequences is called a single nucleotide polymorphism (SNP), and it is detected that they are called SNP detection.
3. Comparative genomic hybridization: applied to identify the increase or decrease of important chromosome fragments carrying disease genes.
Applications of Microarray
Gene Discovery: DNA microarray technology can help identify new genes and understand their function and expression levels under different conditions.
Disease diagnosis: DNA microarray technology helps researchers learn more about different diseases such as heart disease, mental illness, infectious diseases, and particularly cancer research. Until recently, different types of cancer have been classified according to the organ where the tumor occurred. Now, with the development of microarray technology, researchers will be able to further classify cancer types based on patterns of gene activity in tumor cells.
Drug discovery: Microarray technology has a wide range of applications in pharmacogenomics. Pharmacogenomics is the study of the correlation between drug treatment response and the patient's genetic characteristics. Comparative analysis of genes from diseased and normal cells will help identify the biochemical composition of the protein synthesized from the diseased gene. Researchers can use this information to synthesize drugs that interact with these proteins and reduce their effects.
Toxicological research: Microarray technology provides a powerful platform for studying the effects of toxins on the transmission of cells and their offspring. Poison genomics establishes a correlation between the toxic response and changes in the cellular genetic profile of exposure to such toxicants.
About Creative Proteomics
Creative Proteomics owns proteome analysis platform to provide protein separation, characterization, identification and quantification services, featured with high throughput and super-sensitivity. In addition, analyses of protein post-translational modifications such as phosphorylation and glycosylation are available.
Media Contact
Company Name: Creative Proteomics
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Website: https://www.creative-proteomics.com
