MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs in eukaryotes with a length of about 17 ~ 25 BP. Since the discovery of miRNA in 1993, the progress of the small RNA field has subverted our understanding of gene regulation. MiRNA plays an important role in a series of physiological and pathological processes from embryonic development to apoptosis and even tumor growth. MiRNA, which is related to genetics, metabolism, infectious diseases and tumors, provides a new perspective for scientists to carry out pathological research, and is expected to become a reliable biomarker of disease.
Various miRNAs related to inheritance, metabolism, infectious disease and tumor provide a new angle for the pathological study of scientists and are expected to be a reliable biomarker for disease. Scientists are also actively seeking new treatments for disease interventions by changing the function of miRNA and developing new in vivo delivery methods. More about miRNA remain to be explored, and Creative Biogene will walk with you.
Research design
Target miRNA screening: new generation sequencing: SmallRNA sequencing
MiRNA microarray
High-throughput selection of miRNA library
Detection of miRNA and target gene expression level: qPCR
Bioinformatics analysis
MiRNA expression difference analysis
MiRNA target gene prediction
TF-miRNA-mRNA regulatory network
Prediction of related miRNA by UTR region of mRNA
In Vitro experimental functional verification
Functional acquired study: mimic
Functional impairment study: inhibitor
Target gene validation: 3’ UTR reporter gene vector
Functional study of miRNA target gene
Experimental verification in vivo
Animal model construction
High expression of microRNA: agomir
Inhibition of miRNA in vivo: antagomir
Inhibition of siRNA in vivo by chemical modification
Detection of various indexes such as physiology and biochemistry by miRNA target gene
Creative Biogene recommends the perfect miRNA research program to cover the various products and services required from miRNA target to functional validation study.
According to experiment and cycle requirements of customer, we design experiments according to the specific experiment stage or test requirements, and complete the miRNA service of a whole process specified by the customer.
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Company Name: Creative Biogene
Contact Person: Wendy Wilson
Email:Send Email
Phone: 6316197922
Address:45-1 Ramsey Road
City: Shirley
State: New York
Country: United States
Website: https://www.creative-biogene.com
