Abbkine IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labelling.
IFKine™ fluorescence secondary antibodies have high specificity, avoiding the nonspecific hybridization with samples through other species of serum/IgG absorbed. Combined with donkey host, they have good preformance in fluorescence multiple labelling.
IFKine™ fluorophore: Improved brightness and photostability
IFKine™ fluorophores are unique and new generation of fluorescent dye groups, which will help you gain brighter and more stable fluorescence results. During the multiple staining experiments, they exert better effects compared with other commercial dyes as below.
| Fluorophore | Ex/Em(nm) | Superior alternative to |
| IFKine™ Green | 493/518 | FITC, Cy2, Alexa 488, Dylight 488 |
| IFKine™ Orange | 555/570 | Cy3, Rhodamin, Alexa 555, Dylight 549 |
| IFKine™ Red | 591/615 | Texas Red, Alexa 594, Dylight 594 |
IFKine™ secondary antibodies: Donkey host, suitable for multiple labelling
IFKine™ secondary antibodies raised from donkey hosts are compatible with most hosts of primary antibodies, especially suitable for multi-staining. In addition, use donkey serum to block directly, you could avoid the complicated blocking steps, and eliminate the interference of blocking disorder because of different secondary antibodies species.
IFKine™ optimization: Minimum nonspecific binding with samples
IFKine™ secondary antibodies specifically recognize IgG of rabbits, mouses and goats, and they go through the serum absorbtion of many other species, such as hmman, rat, mouse. Experiments demonstrate that the secondary antibodies almost have no cross reactions wiht other serum proteins, avoiding the nonspecific binding with samples, which apply to most species.
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