New York - Jun 29th, 2018 - Creative Bioarray, a leading biotechnology products and service provider, introducing professional primary cell culture guide which help researchers and investigators to learn about the basic principles and process of primary cell culture and overcome the high cost and inconsistency found in culture routine by offering comprehensive solutions. The primary cell culture offered mainly features with a portfolio of flexible and integrated solutions for cell culture including biobank, cell isolation, cell authentication etc.
Primary cells are cells that are isolated directly from human or animal tissue with enzymatic or mechanical methods, the most common primary cells used in the study are epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, myocytes, hematopoietic stem cells, epidermal cells and mesenchymal stem cells. Primary cells can be indefinitely subcultured through an in vitro process called transformation which can occur spontaneously or can be induced by chemical or viral.
Primary cells can be cultured in suspension or adherent cultures. Some cells are naturally present in the suspension and will not adhere to the surface (such as those from peripheral blood). Other cell lines have been modified to survive in suspension cultures, and their growth density is higher than that allowed by adhesion conditions. In establishing the primary cell culture process, antibiotics must be added to the growth medium to inhibit host-organized contamination. It is very important to retain the vitality of primary cells after isolation because most of them undergo the aging process and stop dividing after undergoing a certain number of population doublings. For the long-term viability of cells, excellent cell culture processing skills and appropriate culture conditions will matter.
At present, primary cell culture is increasingly applied as a primary tool for cell and molecular biology to study the normal physiology and biochemistry of cells, such as metabolic studies, aging, signal transduction studies, drugs and toxic compounds on cells and Mutagenesis and carcinogenesis. It is also used for drug screening as well as the development of large-scale biological compounds.
However, there are several challenges in primary cell culture:
Cell contamination - major tissues contamination when transferring to culture medium.
Changes in pH - This may be due to incorrect salt, bacterial or fungal contamination of the medium, insufficient buffering of bicarbonate, incorrect carbon dioxide tension, etc.
Optimal adherence - Adequate or absent adhesion factors in the medium or contaminating or overlying trypsin cells.
Slow growth - Reasons include changes in the pH of the culture medium, necessary growth-promoting ingredient/factor depletion, low contamination, improper reagent storage, etc.
Cell death - fluctuations in temperature, absence of CO2, cell damage during thawing and/or cryopreservation, increased concentrations of toxic metabolites, and imbalance in osmotic pressure in the medium leading to decreased cell survival.
Except for these challenges, the precipitation, cell clumps and induced variability will also influence the cell culture. The application of primary cell culture avoids many moral objections to animal experiments, allowing experiments on human tissues. What’s more, primary cells provide more relevant results than cell lines, pre-screened primary cells can perform as the great model of in vivo signaling.
About Creative Bioarray
Founded in 2005, Creative Bioarray provides consumers with professional biotechnology products and services for the investigation of sciences research facilitation. Creative Bioarray is a well-recognized industry leader with more than ten years experiences that satisfy the needs of our clients across more than 50 countries via cutting-edge products and high-quality services.
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Website: http://www.creative-bioarray.com
