The full-length ORF clone contains only the coding sequence of the full-length protein, while the other full-length cDNA clones contain some untranslated sequences, such as the 5'side or 3' side non translation. It is well known that these untranslated sequences may have a negative effect on the transcription and translation processes of the encoded proteins in the host.
If it is the ORF expressed clone, it can be transfected into cells and expressed in cells.
In the general situation, the carrier of cDNA clone is not the expression vector, so it can not be directly used for transfection of cells.
The difference between ORF, cDNA and CDS:
1.what is a full-length ORF clone?
A full-length ORF clone is a plasmid inserted into a DNA fragment encoding a full-length protein. The inserted DNA fragment only contains sequence incoding a full-length protein, and does not contain the untranslated region of 5 'or 3' end (UTRs) or intron.
2.why should the full length ORF cDNA clones are used instead of other full-length cDNA clones?
The full-length ORF clone contains only the coding sequence of the full-length protein, while the other full-length cDNA clones contain some untranslated sequences, such as the 5'side or 3' side non translation. It is well known that these untranslated sequences may have a negative effect on the transcription and translation processes of the encoded proteins in the host.
ORF sequence: open reading frame. It is suggested that the DNA sequence may be a gene, but the specific gene coding sequence requires additional content. If the promoter and terminator sequence of the DNA sequence are identified, the amino acid information of the sequence can be identified.
cDNA sequence: complementary DNA sequence. It refers to the process mRNA forms DNA under the action of reverse transcriptase. This DNA is cDNA sequence. It has no difference between introns and exons. In prokaryotes, it can be used as a gene sequence encoding polypeptide, but in eukaryotes, due to lack introns, so it should not be used as a gene sequence encoding polypeptide.
CDS sequence: coding sequences, which contain sequences from the start codon to the stop codon.
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