Humanization plays an important role in reducing the immunogenicity of monoclonal antibodies that are derived from xenogeneic sources as well as in improving their activation of the human immune system. As known, rodent antibodies are highly immunogenic in humans, limiting their clinical applications especially when repeated administration is required. Moreover, they are rapidly removed from circulation and can cause systemic inflammatory effects as well.
To solve these problems, Creative Biolabs has developed three antibody humanization strategies that can preserve the specificity and affinity of the antibody toward the antigen whereas significantly or completely eliminate the immunogenicity of the antibody in humans, CDR Grafting & SDR Grafting, Chain Shuffling and Humanized IgG Library Screening.
CDR Grafting & SDR Grafting is featured with randomization of a small set of framework residues utilizing phage display technology and computer modeling. In this platform, six CDR loops comprising the antigen-binding site are grafted into corresponding human framework regions. This approach retains the epitope specificity of the original antibody.
Chain shuffling is an entirely selective humanization strategy based on construction and screening of two chimeric phage display libraries. This approach allows 100% humanization of a mouse antibody. “We would like to propose using our HuScL-2TM Phage Display Naive Human scFv Library with a complexity of 1.42×109 transformants as the backbone of the chimeric libraries and the donor of human VL and VH chains.” said Monika Müller, scientific officer of Creative Biolabs.
Humanized IgG library screeningallows the selection of humanized antibodies in a full-size IgG format that keeps or increases the original affinity of the mouse antibody as well as the selection of high affinity humanized antibodies in a dimeric IgG format. In this method, a mammalian cell surface display library is made to display full-size humanized IgG variants that are to be further selected suing FACS.
“During the past decade, we have performed 15 mouse/rat humanization projects with at least one humanized antibody entered the clinical trials”.
About Creative Biolabs
Creative Biolabs is a professional biotech service provider located in New York. It is specialized in providing custom biotechnology and pharmaceutical services that cover the full scope of biotechnology needs of early drug discovery and development.
Company Name: Creative Biolabs
Contact Person: Candy Swift
Address:45-1 Ramsey Road
State: New York
Country: United States